The Drosophila Dbf4 ortholog Chiffon forms a complex with Gcn5 that is necessary for histone acetylation and viability - Supporting data for Torres-Zelada et al. (2018)

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By Eliana F. Torres-Zelada1, Robert E. Stephenson1, Aktan Alpsoy2, Benjamin D. Anderson1, Selene K. Swanson3, Laurence Florens3, Emily C. Dykhuizen2, Michael P. Washburn4, Vikki Weake5

1. Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA 2. Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA 3. Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, Missouri 64110, USA 4. Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, Missouri 64110, USA, Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA 5. Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA, Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA

Supporting data and protocols for for Torres-Zelada et al. (2018). The Drosophila Dbf4 ortholog Chiffon forms a complex with Gcn5 that is necessary for histone acetylation and viability. Journal of Cell Science.

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Version 1.0 - published on 11 Dec 2018 doi:10.4231/R72V2DD0 - cite this Archived on 08 Feb 2019

Licensed under CC0 1.0 Universal

Complex_Summary_PURR.jpg

Description

A novel histone acetyltransferase complex containing Chiffon and Gcn5 was identified in Drosophila melanogaster: Chiffon Histone Acetyltransferase (CHAT) complex. This dataset contains the mass spectrometry data (excel files), western blots (.scn and tif files), microscopy images (nd2 and tif files), plasmid maps (gbk files) used to generate the figures presented in Torres-Zelada et al. 2018. It also contains R scripts used for generating the box plots, and detailed protocol files for BrdU labeling/immunostaining in ovary follicle cells, immunoprecipitation and mass spectrometry from S2 cells, and histone acetyltransferase assays.

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File Information:

Confocal microscopy images (.nd2, .tif) can be opened using ImageJ or similar software.

Western blot data are provided as tif or .scn files (BioRad ImageLab software).

Plasmid maps are provided in Gene Bank and Clone Manager format (.gbk or .cm5) and can be opened using Notepad.

Mass spectrometry, histone acetylation assays, and histone acetylation quantification (microscopy) are summarized as excel or .csv files.

Scripts used for analysis are provided as .R files and can be opened using RStudio or similar software.

Bio-Rad ImageLab software is available for download at: https://en.freedownloadmanager.org/Windows-PC/Image-Lab-Software.html

The Purdue University Research Repository (PURR) is a university core research facility provided by the Purdue University Libraries, the Office of the Executive Vice President for Research and Partnerships, and Information Technology at Purdue (ITaP).