A novel histone acetyltransferase complex containing Chiffon and Gcn5 was identified in Drosophila melanogaster: Chiffon Histone Acetyltransferase (CHAT) complex. This dataset contains the mass spectrometry data (excel files), western blots (.scn and tif files), microscopy images (nd2 and tif files), plasmid maps (gbk files) used to generate the figures presented in Torres-Zelada et al. 2018. It also contains R scripts used for generating the box plots, and detailed protocol files for BrdU labeling/immunostaining in ovary follicle cells, immunoprecipitation and mass spectrometry from S2 cells, and histone acetyltransferase assays.
Cite this work
Researchers should cite this work as follows:
- Torres-Zelada, E. F., Stephenson, R. E., Alpsoy, A., Anderson, B. D., Swanson, S. K., Florens, L., Dykhuizen, E. C., Washburn, M. P., Weake, V. M. (2018). The Drosophila Dbf4 ortholog Chiffon forms a complex with Gcn5 that is necessary for histone acetylation and viability - Supporting data for Torres-Zelada et al. (2018). Purdue University Research Repository. doi:10.4231/R72V2DD0
Confocal microscopy images (.nd2, .tif) can be opened using ImageJ or similar software.
Western blot data are provided as tif or .scn files (BioRad ImageLab software).
Plasmid maps are provided in Gene Bank and Clone Manager format (.gbk or .cm5) and can be opened using Notepad.
Mass spectrometry, histone acetylation assays, and histone acetylation quantification (microscopy) are summarized as excel or .csv files.
Scripts used for analysis are provided as .R files and can be opened using RStudio or similar software.
Bio-Rad ImageLab software is available for download at: https://en.freedownloadmanager.org/Windows-PC/Image-Lab-Software.html