This data series contains all the raw data and supporting material for the following two publications:

Chen, X., Leon-Salas, W.D., Zigon, T., Ready, D.F. and Weake, V.M. (2017). A programmable optical stimulator for the Drosophila eye. HardwareX 2: 13-33.

Chen, X., Hall, H., Simpson, J. P., Leon-Salas, W. D., Ready, D. F., & Weake, V. M. (2017). Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration. NPJ Aging and Mechanisms of Disease, 3(1). doi:10.1038/s41514-017-0019-6.

This includes the hardware and software files necessary to build the optical stimulator used to subject the flies to blue light stress, and the results (microscopy, biological assays) supporting the mechanism involved in blue light-induced retinal degeneration.

• Drosophila Optical Stimulator (v1.0)

  This publication contains the electronic files required to build an optical stimulator for fruit flies. The stimulator uses red and blue light-emitting diodes (LEDs) and an embedded computer to generate light at different power density levels.

• Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 1 from Chen et al. (2017) (v1.0)

  Supporting microscopy images for Fig 1 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

• A programmable optical stimulator for the Drosophila eye - Supporting biological data for Chen et al. (2017). (v1.0)

  Confocal microscopy images for Chen et al. (2017). HardwareX. "A programmable optical stimulator for the Drosophila eye" 13-33.

• Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 2 and Fig 5 from Chen et al. (2017) (v1.0)

  Supporting confocal microscopy images for Fig 2 and Fig 5 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

• Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 3 (part 1) from Chen et al. (2017) (v1.0)

  Supporting electron microscopy images for Fig 3 (part 1) from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

• Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 3 (part 2) from Chen et al. (2017) (v1.0)

  Supporting electron microscopy images for Fig 3 (part 2) from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

• Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 4 from Chen et al. (2017) (v1.0)

  Supporting confocal and electron microscopy images for Fig 4 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

• Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 6 from Chen et al. (2017) (v1.0)

  Supporting confocal microscopy images and ROS assays for Fig 6 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

• Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for supplemental Figures from Chen et al. (2017) (v1.0)

  Supporting data for supplemental Figures Fig. S1 - Fig. S6 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

Researchers should cite this work as follows:

• Chen, X.; Leon-Salas, W. D.; Hall, H.; Simpson, J. P.; Ready, D. F.; Weake, V. M. (2017). Blue light-induced retinal degeneration in Drosophila melanogaster: Supporting data for Chen et al.. Purdue University Research Repository. doi:10.4231/R789141Q

  BibTex | EndNote

1. Biochemistry
2. Biochemistry (Dept)
3. Blue Light
4. Drosophila
5. Lipid Peroxidation
6. Optical Stimulator
7. Oxidative Stress
8. Phototoxicity
9. Retinal Degeneration

Supporting and raw data are provided for the associated cited studies separated by Figures and/or publications. Supporting data for each figure in Chen et al. studies are published as individual data sets. Information on how to access any specialized file formats are provided in the specific data set. Keys for file names are provided as text files in most figure folders, and describe genotypes/ages/treatments and other experimental variables. For full details of any given experiment, please refer to the published studies (see cited material).