A programmable optical stimulator for the Drosophila eye - Supporting biological data for Chen et al. (2017).

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By Xinping Chen1, Donald F Ready1, Vikki Weake2, Walter Leon-Salas1

1. Purdue University 2. Purdue University, College of Agriculture

Confocal microscopy images for Chen et al. (2017). HardwareX. "A programmable optical stimulator for the Drosophila eye" 13-33.

Version 1.0 - published on 27 Oct 2017 doi:10.4231/R75H7DFT - cite this Archived on 28 Nov 2017

Licensed under CC0 1.0 Universal

Description

A custom optical stimulator was designed to expose Drosophila to defined intensities and duration of blue light, which induces retinal degeneration in flies. The stimulator was tested by exposing Drosophila melanogaster (w1118) to blue or red light at 6 days post-eclosion and assessing retinal degeneration by immunostaining retinas for the rhabdomere markers phalloidin and Rh1. These original confocal microscopy images were used to generate Figure 18 in Chen et al. (2017, HardwareX), and full details are provided in this study.

Full text of the HardwareX paper is available at:
Xinping Chen, Walter D. Leon-Salas, Taylor Zigon, Donald F. Ready, Vikki M. Weake, A programmable optical stimulator for the Drosophila eye, In HardwareX, Volume 2, 2017, Pages 13-33, ISSN 2468-0672, https://doi.org/10.1016/j.ohx.2017.07.001.

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Notes

Confocal microscopy images (.lsm, .tif, .jpg) can be opened using ImageJ or similar software. File names indicate the age, genotype and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. The images used for quantification are provided in the Figure folder corresponding to the appropriate panel in each figure.

ImageJ and LSM-reader plugin are available at:
https://imagej.nih.gov/ij/index.html

https://imagej.nih.gov/ij/plugins/lsm-reader.html

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