Different ages and/or genotypes of Drosophila melanogaster were subjected to blue light stress and assays were performed to examine retinal degeneration (immunostaining retinas for the rhabdomere markers phalloidin and Rh1, confocal microscopy), fatty acid/lipid composition, electroretinograms (ERG) and ROS levels. These data were used to generate supplemental Figures in Chen et al. (2017), and full details citing this study are provided in the accompanying series.

Researchers should cite this work as follows:

• Chen, X., Hall, H., Simpson, J. P., Leon-Salas, W. D., Ready, D. F., Weake, V. M. (2017). Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for supplemental Figures from Chen et al. (2017). Purdue University Research Repository. doi:10.4231/R7NK3C6S

  BibTex | EndNote

1. Biochemistry
2. Drosophila
3. Lipid Peroxidation
4. Phototoxicity
5. Retinal Degeneration

Confocal microscopy images (.lsm, .tif, .jpg) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. ROS assays and lipid analysis are presented as excel or csv files. Electroretinograms (ERGs) are provided as .adicht files (LabChart Data files) and can be opened using LabChart Reader. The data are provided in the Figure folder corresponding to the appropriate panel in each supplemental figure.

ImageJ and LSM-reader plugin are available at:
https://imagej.nih.gov/ij/index.html

https://imagej.nih.gov/ij/plugins/lsm-reader.html

LabChart Reader is available at:

https://www.adinstruments.com/products/labchart-reader