Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for supplemental Figures from Chen et al. (2017)

Listed in Datasets publication by group The Weake lab

By Xinping Chen1, Hana Hall1, Jeffrey Simpson1, Walter Leon-Salas1, Donald Ready1, Vikki Weake2

1. Purdue University 2. Purdue University, College of Agriculture

Supporting data for supplemental Figures Fig. S1 - Fig. S6 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

Version 1.0 - published on 30 Oct 2017 doi:10.4231/R7NK3C6S - cite this Archived on 28 Nov 2017

Licensed under CC0 1.0 Universal

Description

Different ages and/or genotypes of Drosophila melanogaster were subjected to blue light stress and assays were performed to examine retinal degeneration (immunostaining retinas for the rhabdomere markers phalloidin and Rh1, confocal microscopy), fatty acid/lipid composition, electroretinograms (ERG) and ROS levels. These data were used to generate supplemental Figures in Chen et al. (2017), and full details citing this study are provided in the accompanying series.

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Notes

Confocal microscopy images (.lsm, .tif, .jpg) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. ROS assays and lipid analysis are presented as excel or csv files. Electroretinograms (ERGs) are provided as .adicht files (LabChart Data files) and can be opened using LabChart Reader. The data are provided in the Figure folder corresponding to the appropriate panel in each supplemental figure.

ImageJ and LSM-reader plugin are available at:
https://imagej.nih.gov/ij/index.html

https://imagej.nih.gov/ij/plugins/lsm-reader.html

LabChart Reader is available at:

https://www.adinstruments.com/products/labchart-reader

 

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