Different genotypes of Drosophila melanogaster were subjected to blue light stress at different ages (1 or 6 days post-eclosion) and levels of reactive oxygen species were assayed in dissected eyes. Retinal degeneration was assayed in flies supplemented with the antioxidant lipoic acid by immunostaining retinas for the rhabdomere markers phalloidin and Rh1. The ROS assays and original confocal microscopy images were used to generate Figure 6 in Chen et al. (2017), and full details citing this study are provided in the accompanying series.

Researchers should cite this work as follows:

• Chen, X., Hall, H., Simpson, J. P., Leon-Salas, W. D., Ready, D. F., Weake, V. M. (2017). Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 6 from Chen et al. (2017). Purdue University Research Repository. doi:10.4231/R7SB43X3

  BibTex | EndNote

1. Biochemistry
2. Drosophila
3. Lipid Peroxidation
4. Phototoxicity
5. Retinal Degeneration

Confocal and microscopy images (.lsm, .tif) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. ROS assays are provided as excel files. The images used for quantification are provided in the Figure folder corresponding to the appropriate panel in each figure.

ImageJ and LSM-reader plugin are available at:
https://imagej.nih.gov/ij/index.html

https://imagej.nih.gov/ij/plugins/lsm-reader.html