Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 4 from Chen et al. (2017)

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By Xinping Chen1, Hana Hall1, Jeffrey Simpson1, Walter Leon-Salas1, Donald Ready1, Vikki Weake2

1. Purdue University 2. Purdue University, College of Agriculture

Supporting confocal and electron microscopy images for Fig 4 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

Version 1.0 - published on 30 Oct 2017 doi:10.4231/R7X34VNF - cite this Archived on 28 Nov 2017

Licensed under CC0 1.0 Universal

Description

Different genotypes of Drosophila melanogaster were subjected to blue light stress at 6 days post-eclosion and retinal degeneration was assayed by immunostaining retinas for the rhabdomere markers phalloidin and Rh1, and by electron microscopy. These original confocal and electron microscopy images were used to generate Figure 4 in Chen et al. (2017), and full details citing this study are provided in the accompanying series. The genotypes used here are UAS-transgenes expressed under control of the eye-specific Gal4 driver, LGMR-Gal4: UAS-luciferase (control), UAS-HMGCR, and UAS-CYTB5.

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Confocal and electron microscopy images (.lsm, .tif, .jpg) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. The images used for quantification are provided in the Figure folder corresponding to the appropriate panel in each figure.

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