Different genotypes of Drosophila melanogaster were subjected to blue light stress at 6 days post-eclosion and retinal degeneration was assayed by immunostaining retinas for the rhabdomere markers phalloidin and Rh1, and by electron microscopy. These original confocal and electron microscopy images were used to generate Figure 4 in Chen et al. (2017), and full details citing this study are provided in the accompanying series. The genotypes used here are UAS-transgenes expressed under control of the eye-specific Gal4 driver, LGMR-Gal4: UAS-luciferase (control), UAS-HMGCR, and UAS-CYTB5.

Researchers should cite this work as follows:

• Chen, X.; Hall, H.; Simpson, J. P.; Leon-Salas, W. D.; Ready, D. F.; Weake, V. M. (2017). Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 4 from Chen et al. (2017). Purdue University Research Repository. doi:10.4231/R7X34VNF

  BibTex | EndNote

1. Biochemistry
2. Biochemistry (Dept)
3. Drosophila
4. Lipid Peroxidation
5. Phototoxicity
6. Retinal Degeneration

File Information:

Confocal and electron microscopy images (.lsm, .tif, .jpg) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. The images used for quantification are provided in the Figure folder corresponding to the appropriate panel in each figure.

ImageJ and LSM-reader plugin are available at:
https://imagej.nih.gov/ij/index.html

https://imagej.nih.gov/ij/plugins/lsm-reader.html