Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 2 and Fig 5 from Chen et al. (2017)

Listed in Datasets publication by group The Weake lab

By Xinping Chen1, Hana Hall1, Jeffrey Simpson1, Walter Leon-Salas1, Donald Ready1, Vikki Weake2

1. Purdue University 2. Purdue University, College of Agriculture

Supporting confocal microscopy images for Fig 2 and Fig 5 from Chen et al. (2017). "Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration"

Version 1.0 - published on 30 Oct 2017 doi:10.4231/R7F18WWG - cite this Archived on 28 Nov 2017

Licensed under CC0 1.0 Universal

Description

Different genotypes of Drosophila melanogaster were subjected to blue light stress at day 6 post-eclosion and retinal degeneration was assayed by immunostaining retinas for the rhabdomere markers phalloidin and Rh1. These original confocal microscopy images were used to generate Figures 2 and 5 in Chen et al. (2017), and full details citing this study are provided in the accompanying series. The genotypes used here are w1118 (wild-type), ninaE (Rh1 mutant), trp and cyt-b5.

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Confocal microscopy images (.lsm, .tif) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. The images used for quantification are provided in the Figure folder corresponding to the appropriate panel in each figure.

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https://imagej.nih.gov/ij/plugins/lsm-reader.html

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