Different genotypes of Drosophila melanogaster were subjected to blue light stress at day 6 post-eclosion and retinal degeneration was assayed by immunostaining retinas for the rhabdomere markers phalloidin and Rh1. These original confocal microscopy images were used to generate Figures 2 and 5 in Chen et al. (2017), and full details citing this study are provided in the accompanying series. The genotypes used here are w1118 (wild-type), ninaE (Rh1 mutant), trp and cyt-b5.
Cite this work
Researchers should cite this work as follows:
- Chen, X., Hall, H., Simpson, J. P., Leon-Salas, W. D., Ready, D. F., Weake, V. M. (2017). Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 2 and Fig 5 from Chen et al. (2017). Purdue University Research Repository. doi:10.4231/R7F18WWG
Confocal microscopy images (.lsm, .tif) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. The images used for quantification are provided in the Figure folder corresponding to the appropriate panel in each figure.
ImageJ and LSM-reader plugin are available at:
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