Drosophila melanogaster (w1118) were subjected to blue light stress at different ages (1, 3 or 6 days post-eclosion) and retinal degeneration was assayed by immunostaining retinas for the rhabdomere markers phalloidin and Rh1. These original confocal microscopy images were used to generate Figure 1 in Chen et al. (2017), and full details citing this study are provided in the accompanying series.
Cite this work
Researchers should cite this work as follows:
- Chen, X., Hall, H., Simpson, J. P., Leon-Salas, W. D., Ready, D. F., Weake, V. M. (2017). Cytochrome b5 protects photoreceptors from light stress-induced lipid peroxidation and retinal degeneration - Supporting data for Fig 1 from Chen et al. (2017). Purdue University Research Repository. doi:10.4231/R7JS9NMW
Confocal microscopy images (.lsm, .tif) can be opened using ImageJ or similar software. File names indicate the age, genotype, treatment and/or biological replicate as outlined in the text key files for each figure (Fig_key.txt). Quantified data are presented as csv files. The images used for quantification are provided in the Figure folder corresponding to the appropriate panel in each figure.
ImageJ and LSM-reader plugin are available at:
The Weake lab
This publication belongs to the The Weake lab group.