Two novel spliceosomal proteins, SF3B3 and SF3B5, were identified in the SAGA transcriptional coactivator from Drosophila melanogaster. The function of these proteins was characterized using mutations in sf3b5. This dataset contains the original microscopy images, western blotting images and qPCR data used to generate the figures presented in Stegeman et al. 2016 (JMB). It also contains an excel file summarizing the mass spectrometry data described in Figure 1.
Cite this work
Researchers should cite this work as follows:
- Stegeman, R. L., Weake, V. M. (2017). The Spliceosomal Protein SF3B5 is a Novel Component of Drosophila SAGA that Functions in Gene Expression Independent of Splicing - Supporting data for Stegeman et al. (2016). Purdue University Research Repository. doi:10.4231/R74Q7S5J
Table 1 contains mass spectrometry information summary. Plasmid maps are provided as .cm5 (Clone Manager software) and .txt files. Fig4 files contain dissecting microscope images of Drosophila eyes (.tif, .jpeg). Each image represents a single fly of each genotype as indicated by file name. Fig5 files contain original raw qPCR data in Bio-Rad CFX Manager software format (.pcrd) and exported qPCR data as .csv files. Western blots for Fig5 are provided as tif or .scn files (BioRad ImageLab software).
Bio-Rad CFX software is available for download at: http://en.freedownloadmanager.org/Windows-PC/Bio-Rad-CFX-Manager.html
Bio-Rad ImageLab software is available for download at: https://en.freedownloadmanager.org/Windows-PC/Image-Lab-Software.html
The Weake lab
This publication belongs to the The Weake lab group.