The Spliceosomal Protein SF3B5 is a Novel Component of Drosophila SAGA that Functions in Gene Expression Independent of Splicing - Supporting data for Stegeman et al. (2016)

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By Rachel Stegeman1, Vikki Weake2

1. Purdue University 2. Purdue University, College of Agriculture

Supporting and raw data for Figures 1 - 5 from Stegeman R. et al. (2016). The Spliceosomal Protein SF3B5 is a Novel Component of Drosophila SAGA that Functions in Gene Expression Independent of Splicing. Journal of Molecular Biology. PMID: 27185460.

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Version 1.0 - published on 03 Nov 2017 doi:10.4231/R74Q7S5J - cite this Archived on 04 Dec 2017

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Description

Two novel spliceosomal proteins, SF3B3 and SF3B5, were identified in the SAGA transcriptional coactivator from Drosophila melanogaster. The function of these proteins was characterized using mutations in sf3b5. This dataset contains the original microscopy images, western blotting images and qPCR data used to generate the figures presented in Stegeman et al. 2016 (JMB). It also contains an excel file summarizing the mass spectrometry data described in Figure 1.

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File Information:

Table 1 contains mass spectrometry information summary. Plasmid maps are provided as .cm5 (Clone Manager software) and .txt files. Fig4 files contain dissecting microscope images of Drosophila eyes (.tif, .jpeg). Each image represents a single fly of each genotype as indicated by file name. Fig5 files contain original raw qPCR data in Bio-Rad CFX Manager software format (.pcrd) and exported qPCR data as .csv files. Western blots for Fig5 are provided as tif or .scn files (BioRad ImageLab software).

Bio-Rad CFX software is available for download at: http://en.freedownloadmanager.org/Windows-PC/Bio-Rad-CFX-Manager.html
Bio-Rad ImageLab software is available for download at: https://en.freedownloadmanager.org/Windows-PC/Image-Lab-Software.html

 

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